●PARP1 Automodified (PARylated), human
Poly(ADP-ribose) polymerase-1 (PARP1) is an abundant and ubiquitous nuclear enzyme that catalyzes the NAD(+)-dependent addition of ADP-ribose polymers on a variety of nuclear proteins. The Tulip Biolabs, Inc. PARP1 Automodified, Cat. #2095, is highly purified human PARP1, expressed in a baculovirus expression system, to which NAD+ and cofactors have been added to allow PARP automodification by poly(ADP-ribose). The PARP1 is lyophilized from its native state, and upon reconstitution with water, it retains residual poly ADP-ribosylation enzymatic activity.
●Use in Western Blot, 100 ng/lane using HRP/TMB colorimetric methods
●ELISA, 2 ng/well
●SDS-PAGE, 0.75 µg/lane
●As a standard in other assays
Please see the Cat. #2095 Datasheet (pdf) for a complete description.