Cat. #2095

SDS-PAGE/Coomassie blue staining
PARP1 & PARP1 Automodified

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WB Anti-poly(ADP-ribose)
PARP1 & PARP1 Automodified

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PARP1 Automodified (PARylated), human
Poly(ADP-ribose) polymerase-1 (PARP1) is an abundant and ubiquitous nuclear enzyme that catalyzes the NAD(+)-dependent addition of ADP-ribose polymers on a variety of nuclear proteins.  The Tulip Biolabs, Inc. PARP1 Automodified, Cat. #2095, is highly purified human PARP1, expressed in a baculovirus expression system, to which NAD+ and cofactors have been added to allow PARP automodification by poly(ADP-ribose). The PARP1 is lyophilized from its native state, and upon reconstitution with water, it retains residual poly ADP-ribosylation enzymatic activity.

●Use in Western Blot, 100 ng/lane using HRP/TMB colorimetric methods
ELISA, 2 ng/well

●SDS-PAGE, 0.75 µg/lane
●As a standard in other assays

Please see the Cat. #2095 Datasheet (pdf) for a complete description. 

Cat. #2095 PARP1 Automodified









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