Cat. #4301

Isolation from MDCK Cells: PARP1 and Tnks1 

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Isolation of PARsylated PARP1


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Af1521 Macrodomain Poly-ADP-ribose Affinity Resin Set
The Tulip Biolabs, Inc. Poly-ADP-ribose (PAR) Affinity Resin Set is designed for the isolation of poly-ADP-ribosylated (PARylated) proteins from cultured cells and tissue samples.
Through the use of a highly specific macrodomain PAR affinity resin, poly-ADP-ribosylated (PARylated) proteins are isolated from cell or tissue lysates without the use of anti-PAR antibodies.  The resin bound proteins can be eluted from the affinity resin, and analyzed by immunoblotting or other methods.

Cat. #4301 Set contains 1 each of the following (can be ordered separately):

•Poly-ADP-ribose Binding Macrodomain Resin, 1mL (1mg macrodomain fusion protein) supplied as a slurry containing  ~75µL resin, Cat. #2302 

•Poly-ADP-ribose Neg Control Resin, 0.5mL (0.5mg fusion protein) supplied as a slurry containing ~75µL resin, Cat. #2303

The PAR Affinity Resin, Cat. #2302 is highly purified GST-Af1521 macrodomain fusion protein construct expressed in E. coli, and bound to glutathione beads.  The Af1521 macrodomain protein is reported to also bind mono-ADP-ribosylated proteins and ADP-ribose.

PAR Negative Control Resin, Cat. #2303 is identical to the #2302 resin except for two gly to asp substitutions, which abolish PAR binding.  The negative control resin is useful to control for non-specific binding, and its use is optional.

Please see the Cat. #4301 Datasheet (pdf) for a complete description.