●Anti-poly(ADP-ribose), clone 10H, purified IgG
Description: Highly purified mouse IgG with BSA carrier protein as stabilizer (recommended).
Supplied as: 1mg/mL IgG in PBS containing 1%BSA plus a preservative.
Please see the Cat. #1020 Datasheet for a complete description.
Price: Cat. #1020 50µg/$70; 200µg/$200; 1mg/$800
Contact us for custom packaging and larger quantities.
Anti-poly(ADP-ribose), clone 10H, mouse monoclonal antibody
The anti-poly(ADP-ribose) mouse monoclonal antibody, clone 10H, recognizes poly(ADP-ribose). It was developed and originally described by H. Kawamitsu et al. (1984) Biochemistry 23, 3771. Poly(ADP-ribose) is a polymer synthesized by a class of enzymes named poly(ADP-ribose) polymerase (PARP), PARP1 being the canonical member. Using NAD+ as substrate, PARP1 catalyzes the formation of the polymer pADPr, with chain lengths ranging from 2 to 300 residues, containing approximately 2% branching in the chain. pADPr is attached to nuclear and other proteins, and to PARP itself (automodification). The clone 10H monoclonal antibody has been described extensively in the literature, and its uses include Western blotting, immunostaining (see below), ELISA-based PARP activity screen, and immunodot blot.
Anti-poly(ADP-ribose), clone 10H
Our product areas > Poly/mono ADP-ribose Products > Anti-poly(ADP-ribose), clone 10H > NEXT
Also available carrier-protein free (neat), Cat. #1020/N...
●Anti-poly(ADP-ribose), clone 10H, purified IgG, carrier free
Description: Highly purified mouse IgG (same as above), but without BSA carrier.
Supplied as: 1mg/mL IgG in PBS plus a preservative.
Please see the Cat. #1020/N Datasheet for a complete description.
Price: Cat. #1020/N 200µg/$200; 1mg/$800
Contact us for custom packaging and larger quantities.
●Anti-poly(ADP-ribose), clone 10H, biotinylated
Description: Biotinylated purified mouse IgG, clone 10H. This offers additional sensitivity and flexibility compared to the unconjugated IgG when used in conjunction with a variety of streptavidin conjugates.
Supplied as: 0.5mg/mL IgG in PBS containing 1%BSA plus a preservative.
Please see the Cat. #1021 Datasheet for a complete description.
Price: Cat. #1021 100µg/$200; 5 x 100µg/$800
Contact us for custom packaging and larger quantities.
●Anti-poly(ADP-ribose), clone 10H, Agarose
Description: Purified mouse IgG, clone 10H (Cat. #1020) conjugated to CL-agarose via a 10-atom spacer. It is useful for immunoprecipitation and immunoaffinity chromatography purification of PARsylated proteins. The clone 10H monoclonal antibody recognizes poly(ADP-ribose) polymer. The negative control agarose is also available, Cat. #1031, see below.
Supplied as: 1mg of anti-poly(ADP-ribose) clone 10H IgG coupled to 0.5 mL CL-agarose beads in a total volume of 1 mL PBS containing 1%BSA plus a preservative.
Please see the Cat. #1030 Datasheet for a complete description.
Price: Cat. #1030 1mg/$500; 5 x 1mg/$2000
Contact us for custom packaging and larger quantities.
Negative control agarose beads...
●Mouse IgG negative control, Agarose
Description: Highly purified normal mouse IgG conjugated to CL-agarose via a 10-atom spacer, similar to the Cat. #1030 except here uses non-immune mouse IgG. It is useful as a negative control to determine non-specific binding in immunoprecipitation assays compared to Cat. #1030.
Supplied as: 1mg of normal mouse IgG coupled to to 0.5 mL CL-agarose beads in a total volume of 1 mL PBS containing 1%BSA plus a preservative.
Please see the Cat. #1031 Datasheet for a complete description.
Price: Cat. #1031 1mg/$150; 5 x 1mg/$600
Contact us for custom packaging and larger quantities.