Cat. #2090

Inhibition of PARP1 by

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Automodification of PARP1
SDS-PAGE/Coomassie blue

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Automodification of PARP1
WB using anti-PARP1

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PARP1 Enzyme
human, highly active
Poly(ADP-ribose) polymerase-1 (PARP1) is an abundant and ubiquitous nuclear enzyme that catalyzes the NAD(+)-dependent addition of ADP-ribose polymers on a variety of nuclear proteins.  The Tulip Biolabs, Inc. PARP1 Enzyme, Cat. #2090, is highly purified human PARP1, expressed in a baculovirus expression system. The PARP1 has a high specific activity, approx. 20000 U/mg (1U=10 fmol ADP-ribose incorporated into immobilized histone in 30 min @ RT).  It is lyophilized from its native state under special conditions, and upon reconstitution with water, it retains its poly ADP-ribosylation enzymatic activity. Purity by SDS-PAGE is >95%.  Each vial contains 50 µg PARP1 (approx. 1000 Units).

●Use in high-throughput enzyme activity/inhibition assays

●Poly-ADP-ribosylation of target proteins

●Western Blot, 100 ng/lane using HRP/TMB colorimetric methods

●ELISA, 2 ng/well

●SDS-PAGE, 1 µg/lane
●As a standard in other assays

Please see the Cat. #2090 Datasheet (pdf) for a complete description. 

Cat. #2090 PARP1 Enzyme, human